The Revolution of CAR-NK Therapy: Transforming Disease Treatment
The Revolution of CAR-NK Therapy: Transforming Disease Treatment
In the ever-evolving landscape of medical innovation, one breakthrough stands out—a transformative approach known as CAR-NK therapy. This groundbreaking development has the potential to revolutionize disease treatment, offering a safer and more effective solution for patients. Join us as we delve into the five pivotal aspects of CAR-NK therapy, reshaping the future of medical care.
CAR-NK Therapy: Why is it a Game-Changer in Disease Treatment
1. Enhanced Safety and Versatility:
At its core, CAR-NK therapy represents a monumental shift in the treatment of various medical conditions. What sets it apart is its enhanced safety and versatility. Unlike its predecessor, CAR-T therapy, which uses the patient’s own T cells, CAR-NK cells can be derived from healthy donors or stem cells. This distinction significantly reduces the risk of complications, including graft-versus-host disease and cytokine release syndrome, which can sometimes accompany CAR-T therapy. Moreover, CAR-NK cells can be stored and used off-the-shelf, making them more readily available for patients in need.
2. Effective Against a Wide Range of Diseases:
The versatility of CAR-NK therapy extends to its effectiveness against a diverse spectrum of diseases. These remarkable cells possess the ability to target multiple antigens present on various cell types. From hematologic conditions to solid tumors, CAR-NK therapy demonstrates remarkable efficacy. Furthermore, it has the potential to counteract the mechanisms employed by cells to evade the immune system, such as immunosuppression and antigen loss.
3. Cost-Efficiency and Speedy Production:
Another compelling aspect of CAR-NK therapy is its cost-efficiency and rapid production. In comparison to the complex and personalized approach required for CAR-T therapy, CAR-NK cells can be manufactured on a larger scale. This streamlined production process significantly lowers costs, making this innovative therapy more accessible to a wider range of patients. The affordability of CAR-NK therapy brings us one step closer to a future where cutting-edge treatments are within reach for all.
4. A Thriving Field with Promising Potential:
While CAR-NK therapy is still in its early stages of clinical development, the initial results are promising and encouraging. Researchers and medical professionals worldwide are working tirelessly to unlock its full potential. Early trials have demonstrated its safety and efficacy, raising hopes for further breakthroughs in disease treatment.
CAR-NK therapy represents a monumental leap forward in disease treatment. At ISSCA.com, we are dedicated to advancing knowledge in the realm of regenerative medicine. We invite you to explore the transformative possibilities of CAR-NK therapy and its potential to reshape the future of medical care. As we journey together, you’ll discover that the horizon of possibilities in healthcare is continually expanding.
Explore our courses and international congresses at ISSCA.com to deepen your knowledge in regenerative medicine.
- Published in Blog
Beyond Boundaries: How Stem Cells Are Reshaping Organ and Tissue Transplantation
Within the sacred corridors of medical advancement, a profound transformation is silently taking place, poised to reshape the very foundations of organ and tissue transplantation. Envision a realm where donor shortages and the specter of immune system rejection fade into history, and where medical horizons stretch to unprecedented frontiers. In this immersive voyage, we embark on a journey to unmask the enigmatic power of stem cells in the realm of transplantation, a journey that invites you to witness the precipice of medical progress.
Stem Cells: Pioneers of Regeneration
Stem cells, those remarkable architects of the body’s rejuvenation, harbor an astonishing talent for metamorphosis, seamlessly transforming into diverse cell types to mend and invigorate damaged tissues. When seamlessly integrated into the landscape of organ and tissue transplantation, stem cells open a tantalizing path towards surmounting the inherent complexities of these procedures. Whether it’s breathing vitality into faltering heart muscles, reviving the compromised functionality of a beleaguered liver, or revitalizing lungs beset by adversity, stem cells emerge as the harbinger of transformation for transplantation outcomes.
The Choreography of Compatibility
Navigating the intricate choreography of compatibility between donor and recipient is one of the most formidable tasks in transplantation. The ever-watchful immune system often triggers rejection, undermining even the most meticulous transplantation endeavors. Yet, stem cells are orchestrating a symphony of change. Through ingenious techniques, researchers are tapping into stem cells’ immunomodulatory prowess, crafting an environment of harmonious coexistence between donor and recipient. This revolutionary approach not only mitigates the risks of rejection but also broadens the spectrum of viable donors.
A Symphony of Science
The harmony between stem cells and transplantation is not an untested hypothesis; it’s a symphony composed through rigorous scientific exploration. An extensive repertoire of peer-reviewed studies, gracing the pages of prestigious journals such as The Lancet and Nature Communications, underscores the potential of stem cells to elevate transplant success rates. These studies illuminate advancements spanning from refining graft survival to hastening the regeneration of vital tissues, a resounding affirmation of the transformative capabilities underpinning stem cell-driven transplantation.
Embark on a Journey of Revelation
Eager to unravel the mysteries encapsulating stem cell-powered transplantation? We extend a heartfelt invitation to embark on an illuminating expedition through our comprehensive training courses in regenerative medicine. Enrich your understanding under the tutelage of eminent experts, delving into the most recent breakthroughs, evidence-based methodologies, and clinical applications. Step into a realm where the boundaries of medical possibilities are redrawn, where your perception of transplantation is enriched, and where you assume the mantle of a pioneer in the regenerative revolution.
The fusion of stem cells and organ transplantation isn’t confined to the realm of scientific speculation; it represents a monumental stride towards re-scripting the destinies of countless lives. As we venture into an epoch where medical accomplishments redefine our limits, the expedition becomes all the more exhilarating.
Join us at issca.us, where the convergence of science and hope awaits. Immerse yourself in the vanguard of regenerative medicine, where the transformative prowess of stem cells beckons. Together, we shall forge ahead, empowered by the symphony of possibilities, shaping a brighter, healthier future for generations to come.
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Exploring the Frontier of Regenerative Medicine: Unraveling Autologous and Allogeneic Stem Cells
Exploring the Frontier of Regenerative Medicine: Unraveling Autologous and Allogeneic Stem Cells
In the realm of modern medicine, a groundbreaking field has emerged, promising to reshape the way we approach healing and rejuvenation – regenerative medicine. At the heart of this revolution lie stem cells, the unsung heroes of tissue repair and regeneration. In this comprehensive exploration, we delve into the intricate differences between two crucial types of stem cells: autologous and allogeneic. Prepare to be captivated by the scientific intricacies that underpin these extraordinary cells and discover how they’re poised to redefine medical possibilities.
Autologous Stem Cells: A Personalized Touch
Imagine harnessing the power of your body’s own natural healing abilities. Autologous stem cells make this vision a reality. Derived from your own tissues – be it bone marrow, adipose tissue, or other sources – these cells hold the promise of personalized healing. The process involves harvesting the stem cells, processing them to enhance their therapeutic potential, and then reintroducing them into the body. This “self-healing” approach minimizes the risk of rejection and side effects, making autologous stem cell therapies an attractive option for many.
- Scientific Precision:
Autologous stem cell therapies are supported by a wealth of scientific evidence. Studies published in esteemed journals like the Journal of Translational Medicine and Stem Cell Research & Therapy have highlighted their potential in diverse applications, from musculoskeletal disorders to cardiovascular conditions. This approach holds immense promise for targeted healing, while minimizing the risk of immune system complications.
Allogeneic Stem Cells: A Shared Resource for Healing
In the quest for regenerative solutions, allogeneic stem cells emerge as a game-changer. These cells are sourced from carefully screened donors and subsequently administered to patients. By tapping into a shared pool of stem cells, medical practitioners can provide a standardized treatment option that’s readily available. Allogeneic stem cells hold the potential to address a broad spectrum of conditions, offering a versatile and accessible avenue for regenerative therapies.
- Scientific Validation:
The science behind allogeneic stem cell therapies is compelling. Rigorous research published in renowned journals such as the Journal of Stem Cells and Development and Stem Cells Translational Medicine has underscored their efficacy in various medical scenarios. This approach not only broadens the scope of treatment but also expedites the therapeutic process, presenting an exciting frontier in regenerative medicine.
Embrace the Future with Knowledge:
Intrigued by the dynamic interplay of autologous and allogeneic stem cells in regenerative medicine? We invite you to embark on a transformative journey of discovery through our comprehensive training courses. Our platform offers unparalleled insights into the latest advancements, evidence-based practices, and clinical applications of these remarkable stem cells. Guided by esteemed experts in the field, our courses provide a unique opportunity to stay at the forefront of regenerative medicine’s evolution.
As the horizon of medicine expands to encompass regenerative possibilities, autologous and allogeneic stem cells take center stage. These cellular protagonists hold the key to unlocking personalized healing and broad-spectrum rejuvenation, ushering in an era of medical advancement like never before. Join us in our mission to unravel the mysteries of regenerative medicine and explore the profound potential of stem cells. Visit issca.us today, and together, let’s embark on a journey that could reshape the landscape of healthcare for generations to come.
- Published in Blog
Revolutionizing Neurodegenerative Care: The Advancements in Stem Cell Therapy
Neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease and amyotrophic lateral sclerosis, are a group of incurable neurological disorders, characterized by the chronic progressive loss of different neuronal subtypes. The conditions are debilitating and affect millions of people worldwide. Despite its increasing prevalence among the ever-increasing aging population, little progress has been made in the coincident immense efforts towards development of therapeutic agents. However, recent advancements in stem cell therapy including stem cells-derived exosomes, neurotrophic factors (NTFs), and their combination as potential therapeutic agents in neurodegenerative diseases.
In recent years, stem cells have emerged as one of the most active research therapeutic tools for many diseases. Stem cells are undifferentiated cells that have the ability to develop into pluripotent stem cells and adult stem cells. All stem cells have the potentiality of continuous self-renewal, high proliferation, and multidirectional differentiation into various cell types to replace degenerated or dead cells. In the context of neurodegenerative diseases, stem cells can be used to replace lost or damaged neurons, provide neuroprotection, and promote neural repair. These properties make stem cells an attractive option for treating neurodegenerative diseases, as they have the potential to slow or even reverse the progression of these conditions.
One of the key benefits of stem cell therapy for neurodegenerative diseases is that it offers a non-invasive, minimally-invasive, and non-toxic alternative to traditional treatments. Unlike drugs and surgery, stem cell therapy does not produce adverse side effects, and can be administered in a safe and controlled manner. In clinical trials, stem cell therapy has been shown to be effective in reducing symptoms and improving quality of life for patients with neurodegenerative diseases.
- Alzheimer’s disease (AD)
Alzheimer’s disease (AD) is a progressive neurodegenerative disease and the most common form of dementia, affecting approximately 55 million people worldwide. Cognitive impairment is a hallmark of AD. Currently, drug therapies can only delay symptoms, but not relieve disease pathology or progression. Researchers have demonstrated that neurons derived from stem cells can integrate with existing neural networks and repair damaged neurons within the host brain, improving learning and memory deficits, and NTFs can improve AD symptoms and provide neuroprotective effects.
- Parkinson’s disease (PD)
The second most common neurodegenerative disorder is Parkinson’s disease (PD). The motor symptoms of PD mainly include rest tremor, rigidity, bradykinesia and postural instability, while common nonmotor symptoms include neuropsychiatric and sleep disorders as well as sensory and autonomic dysfunction. At present, there is no cure for PD or disease-modifying therapy. It is common for symptom-relief medications to only provide partial relief and elicit side effects such as motor complications, gastrointestinal problems, and neurological issues. In spite of the fact that these treatments do not address the underlying pathology, alternative therapies, especially those based on stem cells and NTFs, are still being pursued intensively.
- Huntington’s disease (HD)
Huntington’s disease (HD) is characterized by motor, cognitive, and psychiatric dysfunctions. The study of multiple possible neurodegenerative mechanisms of HD is currently underway, and this knowledge is expected to contribute to the development of new HD treatments. The abilities of stem cells to rescue or replace the damaged and dying neurons, and to prevent further cell damage and death, make stem cell-based therapies promising for treatment of this neurode-generative disease.
- Amyotrophic lateral sclerosis (ALS)
As a neurodegenerative disorder, amyotrophic lateral sclerosis (ALS) involves progressive degeneration of both upper and lower motor neurons, resulting in palsy and death within 3-5 years of onset. Currently, only two disease-modifying medicines are available, each showing benefit to a limited number of patients. Stem cell-based therapy holds great promise for treating ALS by providing both cell replacement and NTF delivery to target the multiple pathologies. Stem cells available for ALS treatment include NSCs, MSCs, embryonic stem cells, induced pluripotent stem cells, and olfactory ensheathing stem cells.
While stem cell therapy is still in the early stages of development, it holds enormous promise as a future treatment for neurodegenerative diseases. A number of studies have demonstrated that stem cells and NTFs offer considerable therapeutic potential, especially when they are used together. In addition to replenishing target neurons, stem cells combined with NTFs can produce neurotrophins to improve the microenvironment that promotes nerve repair and regeneration. As research continues, we can expect to see even more exciting advancements in this field, bringing hope to those affected by these debilitating conditions.
It’s important to stay informed about the latest developments in stem cell therapy. You can learn more about regenerative medicine and stem cells by enrolling in our international certification program at www.issca.us
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Mesenchymal Stem Cells For Cardiovascular Diseases
Despite progress in cardiovascular research, cardiac pathology continues to be one of the most common causes of morbidity and mortality in the world. Stem cell-based therapy has been recognized as an innovative strategy for the repair, regeneration and functional recovery of the myocardium, hence, once the animal research stage has been overcome, most clinical trials aimed at evaluating the safety and effectiveness of regenerative medicine in cardiovascular diseases have focused on angina pectoris, myocardial infarction and chronic cardiomyopathy. Although the current evidence of benefit is not conclusive, the evidence in favor of favorable results is growing.
In some cases, stem cell therapy can provide an effective treatment or alternative for diseases or disorders for which there is no effective treatment. Because these cells are capable of dividing into a wide range of lineages and tissues, they can be used to treat various diseases by repairing, replacing, and regenerating tissues.
It is unclear how umbilical cord mesenchymal stem cells act on the heart, but previous studies have shown that they possess an anti-apoptotic effect. The induced cardiomyocytes can form discs interspersed with myocytes from the host cells, creating a functional syncytium that will help contract the heart. Mesenchymal stem cells can improve cardiac function and reduce damage caused by cardiovascular disease, since they stimulate endogenous repair mechanisms, the regulation of the immune response, tissue perfusion and the proliferation of the resident heart rate, thereby improving cardiac function and reducing damage severity.
Refractory angina
This syndrome, characterized by persistent angina despite standard medical treatment, is often not revascularized due to diffuse coronary lesions or severe comorbidities.
In 2017, a review included 13 clinical studies, with 1061 patients and 12 months of follow-up on average, indicating cell therapy has emerged as a tool for managing these patients. Although the available data are inconclusive, the authors conclude that stem cell-based therapy could be a viable addition to conventional treatment options for refractory angina, given the paucity of therapeutic alternatives.
There was a reduction in mortality at two years after a meta-analysis in 2018, involving 304 patients, showed improved exercise tolerance and reduced angina attack frequency at three, six and 12 months. An additional meta-analysis published in March 2019, involving 526 patients monitored for 14 months, showed that patients treated with stem cells had fewer serious adverse effects, fewer deaths, fewer angina attacks, and fewer antianginal medications than those treated with conventional management.
A third meta-analysis from 2019, with 269 patients and 15 months of follow-up on average, reports the following results: decreased all-cause mortality, decreased frequency of angina and increased exercise time, with no increase in adverse reactions.
Cardiomyopathy (ischemic and non-ischemic)
Despite optimal medical and surgical management, many patients with heart failure undergo long-term myocardial remodeling that does not allow them to restore their ventricular function. This is because current treatment protocols cannot reverse the loss of cardiomyocytes due to cardiomyopathy. Since inflammatory responses continue over time as a central mechanism in the development of heart failure, it was of interest to investigate the anti-inflammatory, antifibrotic, and immunomodulatory properties of stem cells in patients with ischemic and non-ischemic cardiomyopathy.
In a review of five clinical studies published between 2017 and 2018, including 605 patients, the authors conclude that cell therapy is safe, causes immunomodulatory effects, improves functional capacity, and adds clinical benefits to standard therapies. According to them, the results are promising, and further evidence strengthening is recommended.
Based on a meta-analysis published in May 2019, involving 20 investigations and 1418 patients evaluated for an average of 21 months, stem cells improved cardiac function indicators (LVEF and LVESV), walking distance, functional classification of heart failure, quality of life, and mortality as compared to controls. Hospitalizations and serious adverse events were not different from those in the control group.
In a recent review of 9 studies involving 612 patients with heart failure, improvement was found in clinical and paraclinical parameters, evaluated on average for 9 months. According to the authors, stem cells are an effective therapy for the treatment of heart failure, improving patient prognosis and ability to exercise.
Acute myocardial infarction (AMI)
The application of stem cells was associated with a significant increase in left ventricular ejection fraction (LVEF) and other variables indicative of improved ventricular function and modification of remodeling in patients with AMI receiving timely percutaneous coronary intervention (PCI) and conventional medication in different studies. Even coronary artery bypass grafting with stem cells offers greater benefits. A number of studies have also shown that intravenous administration could be a more efficient and effective method of treating the heart or coronary arteries than direct application, with logistic, safety, and cost advantages.
Stem cell therapy is evidenced to be a safe way to treat cardiovascular diseases, as it shows an anti-apoptotic effect, reduction of lesion size, improvement of cardiac function through regulation of the immune response, adequate tissue perfusion and activation of growth factors.
We still need to explore a lot of ground, in terms of these and other conditions. You can learn more about regenerative medicine and stem cells by enrolling in our international certification program at www.issca.us
- Published in Blog
New advances in osteoarthritis therapy with stem cells
Osteoarthritis is a rheumatic pathology that damages the articular cartilage. By joining two bones through the joint capsule, the joints are able to move, providing us with functional autonomy. An inner fluid called synovial fluid is usually found within joints, which is produced by the synovial membrane. Articular cartilage covers the ends of the bones that form the joint. As a result of damage to this articular cartilage, pain, stiffness, and functional impairment occur. Osteoarthritis is the most common joint disorder, usually beginning between the ages of 40 and 50, affecting to some degree almost everyone over the age of 80. Typically, osteoarthritis affects the spine, shoulders, fingers, hips, knees, and toe joints.
Stem cell therapies have the potential to treat a broad spectrum of diseases. Whether it’s rhizarthrosis, diabetes, neurodegenerative diseases, spinal cord injuries, or heart disease. By utilizing stem cells, regenerative medicine is capable of repairing tissues in affected areas. The main difference between lipo gem therapy and other treatments for osteoarthritis is that lipogem therapy regenerates cartilage, avoids surgery and its sequelae, and improves the quality of life for patients.
The potential for medical treatments with stem cells and their by-products is currently very high. In the field of sports medicine and traumatology, one of the most outstanding advances has been made for the first time in decades recently: Spanish scientists have achieved a degree of tendon regeneration in 100% of injured patients, resulting in a decrease in pain and a return to sport within two months, and just six months after the trial was completed.
A research performed by the Institute of Regenerative Tissue Therapy (ITRT), published by the prestigious American Journal of Sports Medicine. Demonstrates how this therapy regenerates chronic lesions in the patellar tendon and opens up a new therapeutic option for this tissue, which was considered impossible to regenerate.
In most patients, fat tissue can be harvested minimally invasively (under local or general anesthesia), providing a highly viable MSC population regardless of donor age. Similar to MSCs derived from other tissues, adipose tissue-derived MSCs have regenerative potential. As osteoarthritis is a very common joint disease, and knee osteoarthritis is the most common form, it is necessary to review scientific literature on osteoarthritis treatments with stem cells, like lipogems.
Lipogems Therapy
Lipogems therapy is a novel procedure that enhances the body’s natural ability to heal itself through the innovative power of science and biotechnology. The Lipogems method involves injecting mesenchymal stem cells into the joints. Adipose-derived mesenchymal stem cells have enormous regenerative potential. They also have a regenerative capacity independent of their age. Even older individuals can benefit from this procedure.
Injection of mesenchymal stem cells into the knee, particularly in the early stages of osteoarthritis, can stop the process of inflammation and degeneration, especially in the less advanced stages of the disease. In addition to preventing progressive physical deterioration of the articular cartilage, this treatment contributes significantly to a patient’s well-being and prevents the installation of knee prostheses.
Patellar tendinopathy, physiotherapeutic treatment and stem cell therapy
Injuries to the patellar tendon that connects the kneecap to the tibia are known as patellar tendinopathy or patellar tendinitis. The patellar tendon works with the muscles in the front of the thigh to extend the knee so you can kick, run and jump. Athletes who perform frequent jumping in their sports, such as basketball and volleyball, are most likely to suffer from patellar tendonitis. However, people who don’t engage in jumping sports may develop patellar tendonitis. Patients with patellar tendinitis usually begin treatment with physical therapy to stretch and strengthen their knee muscles.
Strength training with eccentric resistance is one of the most common treatments for tendinopathies. Alternatively, it has been demonstrated that bone marrow-derived mesenchymal stem cells (MSCs) can regenerate injured patellar tendons. Within six months of treatment, it has been observed that the structure of this tissue – which is always difficult to treat – is restored, reaching a regeneration of 40% in all injured persons, with a gradual improvement that eventually becomes complete.
It has been found that traditional management methods, including isometric or eccentric exercises, shock wave therapy, and even surgery, are not effective. As part of a rehabilitation program in chronic patellar tendinopathy, autologous expanded bone marrow mesenchymal stem cells (BM-MSC) or leukocyte-poor platelet-rich plasma (Lp-PRP) may be effective in reducing pain and improving activity levels. Traditional management, which includes isometric or eccentric exercises, shock wave therapy, and even surgery, has limited success. A combination of autologous expanded bone marrow mesenchymal stem cells (BM-MSCs) and leukocyte-poor platelet-rich plasma (Lp-PRP) and rehabilitation may reduce pain and improve activity levels in active participants with chronic patellar tendinopathy.
To learn more about stem cells, cellular therapies and keep up to date with all the information about regenerative medicine and its advances, sign up for our international certification in regenerative medicine at www.issca.us
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ISSCA Members to Present in the XXV Congreso Internacional de Medicina, Cirugia Estética y Obesidad
The XXV Congreso Internacional de Medicina, Cirugia Estética y Obesidad will take place in CDMX, México on July 13,14 and 15, 2022. The International Society for Stem Cell Application (ISSCA) will be actively taking part in the congress. The ISSCA is a multidisciplinary community of physicians and scientists with a mission to advance the science, technology, and practice of regenerative medicine to treat diseases and lessen human suffering through science, technology, and regenerative medicine. Several ISSCA members will be presenting or giving demonstrations on the latest protocols and technologies in regenerative medicine.
- Benito Novas, the managing director of the ISSCA from the USA, will give a talk about Digital Marketing in Cash-Based Practices. Benito Novas is a global entrepreneur, manager, and keynote speaker, who specializes in marketing focused on biotechnology, life sciences, and healthcare development. He has served as the director of the ISSCA since 2016. His published books in Aesthetic Practice and Digital Marketing can be found here. In his presentation this July, he will share his visionary approach to healthcare management and regenerative medicine. Congress attendees will have the opportunity to learn why doctors must use digital marketing strategies to grow a successful practice. Benito Novas will provide tips to attendees on how to capitalize on social media trends to grow practice influence.
- Dr. Maritza Novas, the ISSCA director of education from the USA, will talk about Allogeneic Therapies and Stromal Cell Exosomes. The use of allogeneic therapy is one of the most attractive alternatives to autologous products and is of utmost interest to researchers in recent years. Exosomes serve as mediators for cell-to-cell communication and can be used as cell-free therapeutics for their special characteristics. Dr. Maritza Novas has been in aesthetic and anti-aging medicine since 2001. She is globally recognized for her work in regenerative medicine and dedicated service in education.
- Dr. Silvina Pastrana, the Argentina chapter director of the ISSCA from Argentina, will present on Fundamentals of Cellular Therapies and Mesenchymal Stem Cells (MSC). Dr. Pastrana heads a staff of medical specialists in orthopedics, rheumatology, medical clinic, and cosmetic surgery, performing procedures that incorporate stem cell therapies. She has been serving as a staff surgeon for the Hospital Dr. Prof. Luis Güemes for 21 years.
Besides the above presentations, look forward to sessions in the practical portion of the congress, where the following ISSCA members will show the latest protocols and technologies in regenerative medicine:
- Dr. Julio Ferreira (Argentina), Cosmetic Surgery / Aesthetic Medicine
- Dr. Andrea Lapeire (Argentina), Aesthetic Medicine
- Dr. Maritza Novas (USA), ISSCA Director of Education in the USA
- Dr. Silvina Pastrana (Argentina), Chapter Director of ISSCA in Argentina
The ISSCA members are the leaders in setting standards and promoting excellence in the field of regenerative medicine, related education, certification, research, and publications. The Global Stem Cells Groups (GSCG) is a group of companies, including the ISSCA and other members, dedicated to facilitating stem cell research and medicine. Making the benefits of stem cell medicine a reality for both doctors and patients worldwide is the goal of the GSCG. To learn more, visit our sites at the ISSCA and the GSCG.
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How much does exosome therapy cost?
Exosome therapy is the new buzz in the regenerative medicine industry because of how it can repair and regenerate your cells and tissues.
Exosome therapy is safer compared to other cellular therapy because it’s a cell-free therapy with no risk of rejection.
Exosome therapy will be beneficial to you if you’re dealing with conditions such as sport injuries, tissue regeneration, hair loss, erectile dysfunction, chronic pain and so many other applications .
In this article, you’ll be learning the cost of exosome therapy and how you can benefit from exosome therapy.
How the cost of exosome therapy is determined
All cells produce exosomes, which are microvesicles that contain biochemical and genetic information.
Hence, the cost of an exosome product (used in exosome therapy) will depend on what type of cell line (raw tissue source) used to extract the exosomes.
The first factor to determine the cost of an exosome product depends on the quality of the tissue source.
The most commonly used tissue types are cord blood, amniotic fluid and mesenchymal cell cultures.
Exosomes derived from mesenchymal cell cultures are the most difficult to obtain but offer the greatest therapeutic potential.
How much does exosome therapy cost?
The average cost of exosome therapy is $4,900, but the price can be anything from $3,500 – $6,500.
It’s also important to note that the price depends on your specific needs and your treatment plan, as decided by the doctor.
The doctor will schedule a consultation with you to determine your personalized treatment plan.
The exosome therapy can either be given as an IV infusion or as localized injections, depending on the purpose of the therapy.
Exosomes are very useful to revitalize, rejuvenate, restore, and reduce inflammations in the body.
Here are some ways you can benefit from exosome therapy
Hair loss therapy: If you’re in the early stages of hair loss, with exosome therapy you can regenerate your hair whether you’re a man or woman. After exosome therapy, you’ll start seeing new hair growth in as little as two to three months with very significant results showing 6 months or 1 year later.
Chronic pain: In case you’re experiencing chronic pain due to degenerative conditions such as arthritis, exosomes can help to subdue the pain by regenerating the cells and helping the body work better.
Degenerative conditions: If you’re struggling with degenerative medical conditions such as osteoarthritis and musculoskeletal injuries, exosome therapy can help your body repair the damage done to your cells by these conditions, prevent them from getting better, and help you to feel better.
Skin therapy: exosome therapy can reduce inflammation in the skin by improving the strength and elasticity of the skin.
Anti-aging: if you would like to retain your youthful glow, exosome therapy can make you feel young again by rejuvenating your skin due to its ability to reverse the cells dying due to aging.
Where can you get exosome therapy?
Cellular hope institutes provide exosome therapy for patients looking for better outcomes for various conditions.
The exosomes used at Cellular Hope Institute are obtained from umbilical cord tissue that is discarded after a new birth , which means these Exosomes have not been exposed to any contaminating or toxic agent because our cells are as healthy as our body. This gives it a higher capacity to regenerate your cells and tissues.
- Published in Blog
Culture Expanded MSCs
Mesenchymal Stem Cells (MSCs) are the most commonly used cells in stem cell therapy and regenerative medicine, due to their high and multi-potency. Mesenchymal Stem Cells (MSCs) can be isolated from different tissues in the body.
In this article, you’ll be learning about culture-expanded MSCs, how MSCs can be expanded, The potency of MSCs and the type of cells they can differentiate into.
What are culture expanded Mesenchymal Stem cells?
Mesenchymal stem cells are high potent cells used for cellular therapy and isolated from different parts of the body. Mesenchymal stem cells can be used to improve the patient outcome in diseases and conditions such as autoimmune diseases, degenerative diseases, nerve damage, diabetes mellitus, bone problems etc.
For every patient, millions of mesenchymal stem cells are needed and the exact amount varies according to disease, route of administration, administration frequency, weight, and age of patient.
Mesenchymal stem cells are expanded in a culture media, on a large scale in order to obtain the required quantity of cells needed for cellular therapy.
Culture expanded MSCs: How does it work?
Expanding Mesenchymal stem cells in a media involves step by step process of isolation and expansion.
Mesenchymal Stem Cells Isolation
Mesenchymal stem cells can be isolated from different tissues in the human body such as adipose tissues, dental pulp, human bone marrow, umbilical cord tissue, umbilical cord blood, peripheral blood and synovium.
Mesenchymal stem cells are expanded in culture to increase their yield and amplify their desired functions and potency.
Although the population of Mesenchymal Stem Cells obtained will vary from donor to donor, here are some steps to follow:
· Acquire fresh tissue extracts in strictly aseptic conditions, to maintain purity.
· To remove any cell clusters, you have to filter the cell suspension with a 70-mm filter mesh
· Use a centrifuge to roll the cells for about 5 minutes at 500g
· Suspend the cells again the cells to measure the cell viability and yield using Trypan blue exclusion
· Use in T75 culture dishes to culture the cells in 10 mL of complete MSC medium at a density of 25 × 106 cells/mL. You can then go on to Incubate the plates at 37 °C with 5% CO2 in a humidified chamber without any interruption.
· When it’s past 3 h, remove the non-adherent cells that accumulate on the surface of the dish by changing the medium and replacing it with 10 mL fresh complete medium.
· After an additional 8 h of culture, add 10ml fresh complete medium as a replacement for the existing medium. You’ll have to repeat this step every 8 h for up to 72 h of initial culture.
· Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.
Expansion of Mesenchymal Stem Cells in a culture media
Culture expanded mesenchymal cells undergo various stages from the preparation of the culture plate, thawing of Mesenchymal stem cells, and the actual expansion of Mesenchymal stem cells.
The reason behind the cultural expansion of Mesenchymal stem cells is to get them to differentiate into other cell types such as osteoblast, adipocyte, and mesenchymal stromal cells.
In preparation, to expand MSCs in a culture media, you need a culture ware. You can get one plastic or glassware plate and coat it with a sufficient amount of 0.1% gelatin. Don’t forget to aspirate the gelatin solution from the coated plate or flask before you use it.
The next step involves the thawing of the Mesenchymal stem cells, and here are a few steps for you to follow:
After the recommended culture medium and coated culture ware is ready and on standby, remove the vial of Mesenchymal Stem Cells from liquid nitrogen and incubate in a 37C water bath and pay close attention to it, until all the cells are completely thawed. The extent of completely thawed frozen cells and how fast, are what determines the cell viability.
Once the cells have thawed completely, take steps to avoid contamination by disinfecting the walls with 70% ethanol, before you proceed to the next step.
Place the cells in a hood, and carefully transfer the cells to a sterile tube with a pipette (1 or 2ml pipette), Do this in such a way to prevent bubbles.
Then, add drops of Mesenchymal Stem cell expansion medium that have been pre-warmed to 37C to the tube containing the Mesenchymal stem cells.
Be careful to take your time when adding the medium to avoid osmotic shock which could lead to decreased viability.
Proceed to mix the suspension slowly by pipetting up and down two times while avoiding any bubbles.
Place the tube in a centrifuge and centrifuge the tube at 300 x g for 2-3 minutes to roll the cells, and you should not vortex the cells.
After this, then decant as much of the supernatant as possible. These steps are necessary to remove residual cryopreservative (DMSO).
Suspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium again or any alternative of choice, pre-warmed to 37 °C, containing freshly added 8 ng/mL FGF-2 (F0291).
The next step involves placing the cell suspension onto a 10-cm tissue culture plate or a T75 tissue culture flask.
Maintain the cells in a humidified incubator at 37 °C with 5% CO2.
The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2*. Replace with fresh medium containing FGF-2 every two to three days thereafter.
Isolate the cells when they are approximately 80% confluent, using Trypsin-EDTA and passaged further or frozen for later use.
Expansion of Mesenchymal Stem Cells
Once the cells are actively proliferating and have reached a confluence of approximately 80% (before 100%), you should subculture the cells.
Then remove the medium from the 10-cm tissue culture plate containing the confluent layer of human mesenchymal stem cells, carefully and apply 3-5 mL of Trypsin-EDTA Solution, before proceeding to incubate in a 37 °C incubator for 3-5 minutes.
Crosscheck the culture to see if all the cells are completely detached. Then, add 5 mL Mesenchymal Stem Cell Expansion Medium to the plate.
Swirl the plate mildly to mix the cell suspension. Transfer the separated/isolated cells to a 15 mL conical tube.
Centrifuge the tube at 300 x g for 3-5 minutes to pellet the cells.
Throw the supernatant away and apply 2 mL Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2 to the conical tube and completely suspend the cells again. Remember not to vortex the cells.
Then, use a hemocytometer to count the number of cells.
Plate the cells at a density of 5,000-6,000 cells per cm2 into the appropriate flasks, plates, or wells in a Mesenchymal Stem Cell Expansion Medium containing 8 ng/mL FGF-2.
Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.
Functions of Culture Expanded MSCs
Mesenchymal stem cells are required to be expanded in order for them to be used clinically for therapeutic purposes.
The culture expanded MSCs can be induced to differentiate into adipocytes, osteocytes, hepatocytes, chondrocytes, tenocytes and cardiomyocytes.
Because of its potential to differentiate into different kinds of cells in the body, it can be used to manage liver problems, heart problems, joint and bone problems etc.
Mesenchymal stem cells are also used in tissue regeneration and modulation of the immune system. They possess anti apoptotic, angiogenic, anti fibrotic, and anti-oxidative properties.
However, the quantity of MSCs isolated from body tissues is not enough for clinical and therapeutic applications.
This is why MSCs are expanded in culture to increase their yield for desired therapeutic effect.
- Published in Blog
Why cellular therapies have become a standard in clinics that are betting on biological medicine
Cellular therapy is fast becoming a standard therapy in many regenerative clinics today. Many doctors are no longer questioning the safety and effectiveness of stem cell therapy. This is because various stem cell studies are already describing the benefits of stem cells for patients who are living with chronic and autoimmune health conditions.
This article will be talking about why stem cell therapy have become a standard therapy in clinics, the paracrine effect of stem cells, and other reasons why doctors are adopting stem cells in their clinics.
Benefits of stem cell therapy
Stem cell therapy is an important innovation in medicine because of its regenerative power in the human body. Most disease states are characterized by damaged cells, tissues and organs, which is where stem cell therapy comes in. In stem cell therapy, stem cells are administered into the human body and it replaces the cells damaged by disease or health disorders.
Stem cell research has revealed two major ways of using stem cells to rebuild defective and damaged cells. One of these ways can be seen in procedures like bone marrow transplant, where stem cells are used to replace the damaged cells by engraving, and they then differentiate into the proper cell type. Another mechanism relies on the paracrine effect of stem cells. This procedure of stem cell therapy involves using stem cells isolated from a donor to stimulate the patient’s cells to repair damaged tissues.
Additionally, unlike traditional therapy, stem cells have a wide application. Stem cell therapy is used to manage various degenerative diseases, autoimmune disorders, birth defects, and the research is still ongoing for so many other health conditions where stem cells have shown potential.
Also, there is currently a high demand for aesthetic medicine. Stem cell therapy is a proven alternative to other forms of cosmetology such as plastic surgery. Hence, dermatologists are turning to stem cell therapy to administer anti-aging procedures, skin rejuvenation, hair therapy, micro-needling etc.
The Paracrine effect of stem cells
The paracrine effect of stem cells is one of the most outstanding effects of stem cells. It involves using donor cells to stimulate endogenous repair by harnessing the regenerative power of the human body. It is a mechanism of tissue regeneration that has created new possibilities for managing various conditions using stem cell therapy.
The cells that trigger a paracrine response are; mesenchymal cells, umbilical cord blood, umbilical cord tissue, adipose (fat) tissue and blood cells from a donor’s bone marrow.
The paracrine effect occurs when the donor’s cells send the damaged or defective cells signals to induce self regeneration and repair by secreting some factors and proteins. One of the mechanism by which this paracrine effect is initiated, involves the secretion of cytokines and regulatory proteins by the damaged patient’s cells, these cytokines and proteins act as mediators to stimulate an immune response that attracts the donor cells, this causes the donor cells to release proteins and factors that stimulate the patient’s cells to promote cell proliferation, increase vascularization and blood flow to the areas that needs to heal, while reducing inflammation.
Moreover, research has shown that the paracrine effect of stem cells prevents damaged and diseased cells from dying. They are also therapeutically useful in autoimmune diseases and preventing transplant rejection due to the immune suppression effect they have.
Is stem cell therapy effective?
Doctors are always looking for ways to provide the best possible treatment to their patients, and that is why many clinics are embracing stem cell therapy as a standard, due to its many advantages.
Stem cell therapy is one of the most effective and safest therapy patients can receive, when compared to other existing treatment options. Stem cell therapy is used in promoting patient outcomes in a lot of disease conditions that were previously poorly treated by other alternatives.
Again, as new potentials and ways of applying stem cells are being discovered, doctors are beginning to maximize these benefits in their clinics. Some conditions that are currently treated by stem cells include autoimmune conditions, immunotherapy Car-T cells, chronic obstructive pulmonary disease, neurodegenerative conditions, osteoarthritis, spinal cord injury, aesthetics/anti-aging, sports medicine, autism and multiple sclerosis.
Another reason clinics are adopting stem cell therapy as a standard therapy is because it is easy to administer. A lot of machines such as GCELL which makes the harvesting and processing of stem cells easy and fast, have made the procedures easily adaptable by doctors.
Furthermore, stem cell therapy reduces the treatment and recovery time associated with surgical procedures and other treatment options. This alone is a big factor in why stem cells are becoming a standard therapy in clinics.
Therapeutic uses of stem cells vs traditional medicine
Existing stem cell research has shown how the regenerative effect of stem cells is defining the future of medicine. The major advantage of stem cell therapy over conventional medication-based therapy is its safety. Stem cell therapy is aimed at treating the cause of the disease while traditional medicine targets the symptoms.
Another problem with traditional medical therapy is that it introduces another problem while trying to solve the existing one. As a doctor, you always run the risk of causing harm with each prescription because of various adverse effects that could lead to major organ damage of the kidney, liver etc. On the other hand, patients already know this and they are actively seeking better alternatives, this is why stem cell therapy is fast becoming a standard therapy in clinics.
Moreover, doctors will always be concerned about whether their patients are taking their medications or not. The burden of drug compliance and adherence associated with traditional medical therapy is not always easy to navigate. This is why effective treatment options like stem cell therapy have become a standard therapy in clinics. It only requires the patients having a procedure that repairs and restores damaged cells and tissues in the most natural way.
If you would like to become certified in regenerative medicine using stem cells and other cellular therapy, contact us.
- Published in Blog