Culture Expanded MSCs
Mesenchymal Stem Cells (MSCs) are the most commonly used cells in stem cell therapy and regenerative medicine, due to their high and multi-potency. Mesenchymal Stem Cells (MSCs) can be isolated from different tissues in the body.
In this article, you’ll be learning about culture-expanded MSCs, how MSCs can be expanded, The potency of MSCs and the type of cells they can differentiate into.
What are culture expanded Mesenchymal Stem cells?
Mesenchymal stem cells are high potent cells used for cellular therapy and isolated from different parts of the body. Mesenchymal stem cells can be used to improve the patient outcome in diseases and conditions such as autoimmune diseases, degenerative diseases, nerve damage, diabetes mellitus, bone problems etc.
For every patient, millions of mesenchymal stem cells are needed and the exact amount varies according to disease, route of administration, administration frequency, weight, and age of patient.
Mesenchymal stem cells are expanded in a culture media, on a large scale in order to obtain the required quantity of cells needed for cellular therapy.
Culture expanded MSCs: How does it work?
Expanding Mesenchymal stem cells in a media involves step by step process of isolation and expansion.
Mesenchymal Stem Cells Isolation
Mesenchymal stem cells can be isolated from different tissues in the human body such as adipose tissues, dental pulp, human bone marrow, umbilical cord tissue, umbilical cord blood, peripheral blood and synovium.
Mesenchymal stem cells are expanded in culture to increase their yield and amplify their desired functions and potency.
Although the population of Mesenchymal Stem Cells obtained will vary from donor to donor, here are some steps to follow:
· Acquire fresh tissue extracts in strictly aseptic conditions, to maintain purity.
· To remove any cell clusters, you have to filter the cell suspension with a 70-mm filter mesh
· Use a centrifuge to roll the cells for about 5 minutes at 500g
· Suspend the cells again the cells to measure the cell viability and yield using Trypan blue exclusion
· Use in T75 culture dishes to culture the cells in 10 mL of complete MSC medium at a density of 25 × 106 cells/mL. You can then go on to Incubate the plates at 37 °C with 5% CO2 in a humidified chamber without any interruption.
· When it’s past 3 h, remove the non-adherent cells that accumulate on the surface of the dish by changing the medium and replacing it with 10 mL fresh complete medium.
· After an additional 8 h of culture, add 10ml fresh complete medium as a replacement for the existing medium. You’ll have to repeat this step every 8 h for up to 72 h of initial culture.
· Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.
Expansion of Mesenchymal Stem Cells in a culture media
Culture expanded mesenchymal cells undergo various stages from the preparation of the culture plate, thawing of Mesenchymal stem cells, and the actual expansion of Mesenchymal stem cells.
The reason behind the cultural expansion of Mesenchymal stem cells is to get them to differentiate into other cell types such as osteoblast, adipocyte, and mesenchymal stromal cells.
In preparation, to expand MSCs in a culture media, you need a culture ware. You can get one plastic or glassware plate and coat it with a sufficient amount of 0.1% gelatin. Don’t forget to aspirate the gelatin solution from the coated plate or flask before you use it.
The next step involves the thawing of the Mesenchymal stem cells, and here are a few steps for you to follow:
After the recommended culture medium and coated culture ware is ready and on standby, remove the vial of Mesenchymal Stem Cells from liquid nitrogen and incubate in a 37C water bath and pay close attention to it, until all the cells are completely thawed. The extent of completely thawed frozen cells and how fast, are what determines the cell viability.
Once the cells have thawed completely, take steps to avoid contamination by disinfecting the walls with 70% ethanol, before you proceed to the next step.
Place the cells in a hood, and carefully transfer the cells to a sterile tube with a pipette (1 or 2ml pipette), Do this in such a way to prevent bubbles.
Then, add drops of Mesenchymal Stem cell expansion medium that have been pre-warmed to 37C to the tube containing the Mesenchymal stem cells.
Be careful to take your time when adding the medium to avoid osmotic shock which could lead to decreased viability.
Proceed to mix the suspension slowly by pipetting up and down two times while avoiding any bubbles.
Place the tube in a centrifuge and centrifuge the tube at 300 x g for 2-3 minutes to roll the cells, and you should not vortex the cells.
After this, then decant as much of the supernatant as possible. These steps are necessary to remove residual cryopreservative (DMSO).
Suspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium again or any alternative of choice, pre-warmed to 37 °C, containing freshly added 8 ng/mL FGF-2 (F0291).
The next step involves placing the cell suspension onto a 10-cm tissue culture plate or a T75 tissue culture flask.
Maintain the cells in a humidified incubator at 37 °C with 5% CO2.
The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2*. Replace with fresh medium containing FGF-2 every two to three days thereafter.
Isolate the cells when they are approximately 80% confluent, using Trypsin-EDTA and passaged further or frozen for later use.
Expansion of Mesenchymal Stem Cells
Once the cells are actively proliferating and have reached a confluence of approximately 80% (before 100%), you should subculture the cells.
Then remove the medium from the 10-cm tissue culture plate containing the confluent layer of human mesenchymal stem cells, carefully and apply 3-5 mL of Trypsin-EDTA Solution, before proceeding to incubate in a 37 °C incubator for 3-5 minutes.
Crosscheck the culture to see if all the cells are completely detached. Then, add 5 mL Mesenchymal Stem Cell Expansion Medium to the plate.
Swirl the plate mildly to mix the cell suspension. Transfer the separated/isolated cells to a 15 mL conical tube.
Centrifuge the tube at 300 x g for 3-5 minutes to pellet the cells.
Throw the supernatant away and apply 2 mL Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37 °C) containing 8 ng/mL FGF-2 to the conical tube and completely suspend the cells again. Remember not to vortex the cells.
Then, use a hemocytometer to count the number of cells.
Plate the cells at a density of 5,000-6,000 cells per cm2 into the appropriate flasks, plates, or wells in a Mesenchymal Stem Cell Expansion Medium containing 8 ng/mL FGF-2.
Cells can be frozen in MSC growth media plus 10% DMSO (D2650) at a density of 2X106 cells/vial.
Functions of Culture Expanded MSCs
Mesenchymal stem cells are required to be expanded in order for them to be used clinically for therapeutic purposes.
The culture expanded MSCs can be induced to differentiate into adipocytes, osteocytes, hepatocytes, chondrocytes, tenocytes and cardiomyocytes.
Because of its potential to differentiate into different kinds of cells in the body, it can be used to manage liver problems, heart problems, joint and bone problems etc.
Mesenchymal stem cells are also used in tissue regeneration and modulation of the immune system. They possess anti apoptotic, angiogenic, anti fibrotic, and anti-oxidative properties.
However, the quantity of MSCs isolated from body tissues is not enough for clinical and therapeutic applications.
This is why MSCs are expanded in culture to increase their yield for desired therapeutic effect.
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Why cellular therapies have become a standard in clinics that are betting on biological medicine
Cellular therapy is fast becoming a standard therapy in many regenerative clinics today. Many doctors are no longer questioning the safety and effectiveness of stem cell therapy. This is because various stem cell studies are already describing the benefits of stem cells for patients who are living with chronic and autoimmune health conditions.
This article will be talking about why stem cell therapy have become a standard therapy in clinics, the paracrine effect of stem cells, and other reasons why doctors are adopting stem cells in their clinics.
Benefits of stem cell therapy
Stem cell therapy is an important innovation in medicine because of its regenerative power in the human body. Most disease states are characterized by damaged cells, tissues and organs, which is where stem cell therapy comes in. In stem cell therapy, stem cells are administered into the human body and it replaces the cells damaged by disease or health disorders.
Stem cell research has revealed two major ways of using stem cells to rebuild defective and damaged cells. One of these ways can be seen in procedures like bone marrow transplant, where stem cells are used to replace the damaged cells by engraving, and they then differentiate into the proper cell type. Another mechanism relies on the paracrine effect of stem cells. This procedure of stem cell therapy involves using stem cells isolated from a donor to stimulate the patient’s cells to repair damaged tissues.
Additionally, unlike traditional therapy, stem cells have a wide application. Stem cell therapy is used to manage various degenerative diseases, autoimmune disorders, birth defects, and the research is still ongoing for so many other health conditions where stem cells have shown potential.
Also, there is currently a high demand for aesthetic medicine. Stem cell therapy is a proven alternative to other forms of cosmetology such as plastic surgery. Hence, dermatologists are turning to stem cell therapy to administer anti-aging procedures, skin rejuvenation, hair therapy, micro-needling etc.
The Paracrine effect of stem cells
The paracrine effect of stem cells is one of the most outstanding effects of stem cells. It involves using donor cells to stimulate endogenous repair by harnessing the regenerative power of the human body. It is a mechanism of tissue regeneration that has created new possibilities for managing various conditions using stem cell therapy.
The cells that trigger a paracrine response are; mesenchymal cells, umbilical cord blood, umbilical cord tissue, adipose (fat) tissue and blood cells from a donor’s bone marrow.
The paracrine effect occurs when the donor’s cells send the damaged or defective cells signals to induce self regeneration and repair by secreting some factors and proteins. One of the mechanism by which this paracrine effect is initiated, involves the secretion of cytokines and regulatory proteins by the damaged patient’s cells, these cytokines and proteins act as mediators to stimulate an immune response that attracts the donor cells, this causes the donor cells to release proteins and factors that stimulate the patient’s cells to promote cell proliferation, increase vascularization and blood flow to the areas that needs to heal, while reducing inflammation.
Moreover, research has shown that the paracrine effect of stem cells prevents damaged and diseased cells from dying. They are also therapeutically useful in autoimmune diseases and preventing transplant rejection due to the immune suppression effect they have.
Is stem cell therapy effective?
Doctors are always looking for ways to provide the best possible treatment to their patients, and that is why many clinics are embracing stem cell therapy as a standard, due to its many advantages.
Stem cell therapy is one of the most effective and safest therapy patients can receive, when compared to other existing treatment options. Stem cell therapy is used in promoting patient outcomes in a lot of disease conditions that were previously poorly treated by other alternatives.
Again, as new potentials and ways of applying stem cells are being discovered, doctors are beginning to maximize these benefits in their clinics. Some conditions that are currently treated by stem cells include autoimmune conditions, immunotherapy Car-T cells, chronic obstructive pulmonary disease, neurodegenerative conditions, osteoarthritis, spinal cord injury, aesthetics/anti-aging, sports medicine, autism and multiple sclerosis.
Another reason clinics are adopting stem cell therapy as a standard therapy is because it is easy to administer. A lot of machines such as GCELL {Insert link} which makes the harvesting and processing of stem cells easy and fast, have made the procedures easily adaptable by doctors.
Furthermore, stem cell therapy reduces the treatment and recovery time associated with surgical procedures and other treatment options. This alone is a big factor in why stem cells are becoming a standard therapy in clinics.
Therapeutic uses of stem cells vs traditional medicine
Existing stem cell research has shown how the regenerative effect of stem cells is defining the future of medicine. The major advantage of stem cell therapy over conventional medication-based therapy is its safety. Stem cell therapy is aimed at treating the cause of the disease while traditional medicine targets the symptoms.
Another problem with traditional medical therapy is that it introduces another problem while trying to solve the existing one. As a doctor, you always run the risk of causing harm with each prescription because of various adverse effects that could lead to major organ damage of the kidney, liver etc. On the other hand, patients already know this and they are actively seeking better alternatives, this is why stem cell therapy is fast becoming a standard therapy in clinics.
Moreover, doctors will always be concerned about whether their patients are taking their medications or not. The burden of drug compliance and adherence associated with traditional medical therapy is not always easy to navigate. This is why effective treatment options like stem cell therapy have become a standard therapy in clinics. It only requires the patients having a procedure that repairs and restores damaged cells and tissues in the most natural way.
If you would like to become certified in regenerative medicine using stem cells and other cellular therapy, contact us.
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Does Stem Cell Therapy Help With Osteoarthritis?
Overview
Joint pain is one of the most common complaints in medicine. It is more prevalent in seniors whose joints have been subject to long-term wear and tear.
Many people develop conditions like osteoarthritis (RA) and rheumatoid arthritis (RA). The former is more common in women.
Although the pain may be temporary, it can also last for months or even years. For this reason, researchers suggested stem cell therapy to address joint disorders.
In this article, we will discuss how stem cell therapy can be useful in treating joint conditions such as osteoarthritis.
Stem cell therapy and arthritis
According to the Centers for Disease Control and Prevention, 54.4 million Americans (22.7%) suffered from arthritis during 2013–2015.
The number is likely to be underestimated because people don’t always seek medical attention. Instead, they prefer to use home remedies to soothe the pain.
These statistics are still extremely troubling because of the poor functional prognosis associated with arthritis.
Once you get diagnosed with arthritis, several treatments could be prescribed, including acetaminophen – it is generally the first-line treatment.
Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are the second. These include naproxen, diclofenac, and ibuprofen.
Side effects of NSAIDs are:
- Stomach ulcers
- Mineral deficiencies
- Increased bleeding risk
Corticosteroids (e.g., prednisone) are another alternative to NSAIDs. These drugs get used when NSAIDs fail to work. Side effects of drugs such as prednisone can be very serious, presenting as:
- Cushing syndrome
- High blood sugar
- Osteoporosis
- Immunosuppression
Non-pharmacological treatments such as cryotherapy or thermotherapy are the final options for treating joint problems.
How can cell therapy help with arthritis
Many treatment options for joint disorders can cause serious side effects, which may be more severe than the original symptoms. Therefore, scientists are always looking for alternative therapies that could help patients without causing an array of side effects.
Enter: Stem Cell Therapy
Patients with osteoarthritis often undergo joints tissue degeneration. Their articulations start to lack structural elements, including bone, cartilage, and ligaments. From this concept, the idea of using stem cell therapy to treat arthritis originated.
Because stem cells have the ability to differentiate into any cell type, they could replace the lost tissue and help restore joint function.
While this treatment may not cure all joint disorders, it is still a viable option that should be discussed. The need for stem cell therapy becomes more pronounced when your symptoms do not respond to conventional therapies.
The best part about stem cell therapy is its side effect profile. Unlike other treatments, stem cell therapy barely causes any adverse effects. In other words, it is completely safe to use on almost everyone.
Here you can check more information about Osteoarthritis and our treatments: https://cellularhopeinstitute.com/osteoarthritis/
Takeaway message
Many medical institutions are now incorporating stem cell therapy in their treatment plans. Orthopedic surgery is one of the most popular.
Hopefully, the use of stem cell therapy will become more mainstream to help as many patients as possible.
If you want to learn more about stem cell therapy, please do not hesitate to check out this link https://cellularhopeinstitute.com/
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Exosome Therapy – The Secret Treatment of Anti-Aging
Overview – The Secret Treatment of Anti-Aging
Over the past few years, exosomes had made a name in the field of rejuvenation and antiaging. Based on the available data, it seems that exosomes will be an integral part of rejuvenation therapy, especially in people interested in stem cell therapy.
Unsurprisingly, many people have never heard of exosomes. So, what exactly are exosomes? How would they help with aging? And what is the difference between exosome therapy and stem cell therapy?
In this article, we will answer all of these questions to help you understand the potential role of this innovative technique.
What are exosomes?
Inside your cells, there are several components that serve different functions. We call them organelles. When these components fuse with the membrane of the cell, exosomes form.
Close inspection of these exosomes shows that they contain protein, lipids, RNA, and other elements of the hosting cell.
Using this complex physiological concept, researchers used stem cell-derived exosomes to repair different tissues. The unique blend of components found inside of these vesicles makes exosomes very effective in boosting the body’s own regenerative ability.
Moreover, exosomes help with getting rid of cytotoxins, making them very effective in dampening chronic inflammation.
How will exosome therapy help your skin
As we age, our skin becomes prone to age-related deterioration, such as wrinkles. Primarily, these issues originate when you can’t regenerate the lost components of your skin.
The good news is that exosomes might just be the solution you’ve been looking for. This treatment will delay the aging process and promote skin regeneration.
When studied in the laboratory, exosomes showed some impressive effects in delaying the onset of skin aging. Researchers found that this therapy boosts the development of fibroblasts, which are a special type of cells that ensure your skin’s structural integrity and elasticity.
Today, the topical use of exosome therapy can be a fantastic way to slow down the dermatological effects of aging.
Perhaps the best thing about exosomes is the fact that there is no risk of rejection since they are not real tissues but rather a trigger for your own tissues to heal themselves.
What is the difference between stem cell therapy and exosomes?
Stem cell therapy revolves around using whole cells to replace lost tissue. Conversely, exosomes are vesicles that detach from the cells. The way that exosomes help with rejuvenation is by helping other cells using the components carried in the vesicles.
Another key difference between stem cells and exosomes is that the former is only obtained from specific areas of the body, such as:
- Bone marrow
- Blood
- Fat tissue
On the other hand, you can get exosomes from all types of cells.
Takeaway message
The use of exosomes in the field of rejuvenation therapy revealed very promising results in preliminary research studies. If used properly, the clinical applications of this technique could be vast, extending beyond esthetic aspects.
We hope that this article helped you appreciate the role of exosome therapy in addressing the aging process.
Learn more about exosome therapy and the possibility to receive it in Cancun, México by clicking on this link.
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The Potential Benefits of Stem Cells for Concussions – What You Need To Know (2021)
Overview
Traumatic brain injuries (TBI), also known as concussions, are among the most frequent causes of death in the United States.
According to the Centers for Disease Control and Prevention (CDC), the number of emergency department visits related to TBI admissions, complications, and deaths increased by 53% in recent years.
People who can recover from TBI may experience irreversible neurological issues throughout their lives. As a result, they lose their autonomy and become reliant on other people to perform daily activities.
However, it’s not all doom and gloom. A few studies suggest that stem cell therapy could be beneficial in the management of TBI.
In this article, we’ll discuss everything you need to know about the potential effects of stem cell therapy in managing concussions.
How can stem cells help with concussions
For those unfamiliar with how stem cells function, here is a short description:
Stem cells can develop into any kind of tissue or cell, including those that don’t typically regenerate (e.g., heart cells, nerve cells). For instance, medical schools often teach that a person has the same number of neurons from the moment they are born to their death.
Although this assertion is not completely true due to the latest evidence that supports the ability of neurons to regenerate, the rate of regeneration is nowhere near enough to restore the damaged tissues caused by TBI or strokes.
To this end, researchers are using stem cells to create new neurons to help patients with TBI, multiple sclerosis, and other neurological disorders.
What is the scientific consensus on stem cell therapy and traumatic brain injuries?
Mesenchymal stem cells derived from the bone marrow, umbilical cord, or adipose tissues showed positive results in the management of concussions.
In a number of studies, the administration of stem cell therapy via the spinal cord showed real evidence of lesion improvement. Researchers used advanced imaging techniques such as functional MRI to objectify the changes.
Neuroscientists theorize that stem cells can replace the dead neurons, which helps with restoring some of the lost functions.
Today, hundreds of clinical trials are trying to unveil the full extent of how stem cell therapy helps with traumatic brain injuries.
Based on the available research, scientists advocate for the use of stem cell therapy in treating patients with severe concussions.
However, neuroscientists insist that stem cell therapy is only used in patients who failed to respond to conventional treatment methods.
To see whether you are a candidate for stem cell therapy, contact us by clicking on this link. You will speak with one of our healthcare professionals in Cancun, México.
Takeaway message
Stem cells have an amazing ability to divide into any type of tissue and potentially restore lost functions.
We hope that this article managed to highlight the potential role of stem cell therapy in addressing traumatic brain injury.
If you have any questions or concerns regarding stem cell therapy or TBI, please do not hesitate to share your thoughts in the comment section below.
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Can Stem Cell Therapy Help With Alopecia (hair loss)?
Can Stem Cell Therapy Help With Alopecia (hair loss)?
Despite the impressive advancements made in the field of medicine, hair loss remains unsolved today.
Every year, hundreds of clinical trials attempt to come up with a revolutionary treatment that will restore hair loss. Unfortunately, most of these studies fail to produce sufficient results.
Interestingly, the issue with hair loss is not financial. In other words, the scientific community spent billions of dollars to find a solution for this problem. This is not the case for other dermatological conditions (e.g., rosacea).
In this article, we will briefly cover the downsides of hair loss, then switch gears to the potential effects of stem cell therapy in addressing this issue.
The downsides of hair loss
While unaffected people see hair loss as a mere esthetic issue, patients with this condition experience many adverse effects, including mental health problems, such as depression, low self-esteem, and a high sense of self-consciousness.
These issues could negatively impact the quality of life of these patients.
Moreover, alopecia affects both genders in great numbers, making this is a real public health issue. We should note that some cases of hair loss have underlying conditions, such as hypothyroidism, autoimmune diseases, and infections
Stem cell therapy and hair loss
In recent years, the clinical uses of stem cell therapy have expanded, covering many fields in medicine, such as hair loss.
The primary issue with treating chronic illnesses is their irreversible nature. In other words, once the tissue is lost, there is no effective medication that can recover it.
For instance, when the cells responsible for producing hair follicles die, no drug will be able to restore them.
This is the core issue with alopecia.
Fortunately, biological treatments such as stem cell therapy and exosome therapy (insert link) might just be the solution to this issue. Due to the incredible ability of stem cells to differentiate into any cell type, they could replace the cells responsible for producing hair follicles.
Typically, your doctor will extract stem cells from your own bone marrow or adipocytes (i.e., fat cells) to prevent any immunological reactions.
Using all of this information, some clinics started offering stem cell therapy to manage hair loss, using noninvasive procedures.
Usually, your doctor will schedule several sessions in order for the results to become apparent. However, many patients report positive findings after the very first therapeutic session.
Despite the underexploited nature of stem cell therapy, it may potentially help with numerous medical fields, including dermatology and esthetic medicine.
Takeaway message
Hair loss is a real issue for people from all across the world. Treating this disorder is really challenging, especially when the underlying condition is unclear. Fortunately, stem cell therapy may play an important role in addressing this issue since it’s one of the few therapeutic modalities that actually restore lost tissue.
We hope that this article helped you appreciate the role of stem cell therapy in the management of alopecia.
If you want to become a specialist in stem cell therapies and help your patients, check our next training dates here
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Wharton’s Jelly injection
Wharton’s Jelly injection
Wharton’s Jelly is the gelatinous connective tissue that is found in the umbilical cord. This once discarded substance that was once thought of as after birth waste, is chocked full of healing substances and abundant in mesenchymal stem cells (MSCs).
The mesenchymal stem cells that are found in Wharton’s Jelly are special in that they are not readily recognized by the body’s immune system. They are considered “primitive cells,” meaning they contain similar properties to embryonic cells. This means that when mesenchymal stem cells derived from Wharton’s Jelly are injected into the patient, there is a reduced risk they will have an immune reaction to the cells.
Minimally processed Wharton’s Jelly preserves the growth factors and proteins needed for effective healing.
Who can benefit from Wharton’s Jelly Treatment?
Wharton’s Jelly is a component used in regenerative medicine, that is medicine that uses naturally occurring cells and substances to promote regeneration of tissue and healthy cells. This natural substance contains collagen, anti-inflammatory properties, growth protein as well as hyaluronic acid.
This means that treatment with Wharton’s Jelly can help with a variety of ailments including degenerative diseases, osteoarthritis, ligament and muscle damage, joint pain and inflammation, chronic pain, as well as promote wound healing.
There are no age restrictions on who can receive this treatment and oftentimes a patient will only need treatment once.
How does Wharton’s Jelly Treatment Work?
A specialized, minimally processed product derived from the Wharton’s Jelly is injected into the injured site. While numbing is not always needed, at times lidocaine will be used for particularly sensitive areas. This process is a relatively painless pinch of a needle. Once the Wharton’s Jelly substance is injected, the cells get to work. The mesenchymal stem cells begin to transform into the cells that are needed to regenerate damaged tissue. This inturn reduces inflammation and pain and returns functionality to the body.
Are there Any Side Effects to the Treatment?
No long-term risks or side effects have been reported. It is important to talk with your doctor about proper protocols around treatment.
Reasons to Undergo Cellular Therapy in Cancun, Mexico with us
Chief among the many advantages that come with receiving your cellular therapy treatment in Mexico, there is the possibility to cultivate and reproduce, in a laboratory environment, those cells which have been extracted from patients. That is to say that a patient can be administered a much larger concentration of cells, which leads to improved results.
Additionally, Mexico is a wonderful option for those treatments that require a more invasive approach– a larger operating team, more medical products and PPE. Mexico is one of the Western Hemisphere’s largest medical tourism capitals, which means that treatments there are performed in hospitals that comply with FDA regulations, and are offered at a more affordable price point.
One can imagine that these types of treatments require a large investment on the side of the patient, however, the cost of a multiple-day cellular therapy treatment in Mexico, is far more affordable than the same treatment– that is, without the ability to use culture-expanded cells, in the United States and Canada.
What is the Recovery Period?
The actual injection will not cause the need for a recovery period. Most patients can go right back to their normal day activities. In cases of injury to ligaments or joints, rest of the injury may be required. Please follow your doctor’s advice for best results from treatment.
Overall Wharton’s Jelly injection treatment is minimally invasive and can help with a variety of ailments. With it’s limited side effects and regenerative properties, it is safe for patients of any age.
Talk with your us to see if Wharton’s Jelly injection therapy is the right fit for you (Clic here)
Cellular Hope Institute
At Cellular Hope Institute, we evaluate, diagnose, and treat patients around the world on a daily basis, with the latest regenerative medicine modalities available today.
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Medical applications of exosomes
Medical applications of exosomes
Exosome therapy is gaining popularity in regenerative medicine. So what exactly are exosomes? Exosomes are particles that are released but cannot be replicated. They are intrical in how cells talk to each other. One cell will have a bunch of vesicles that leave and head to another cell to deliver a “message.” These vesicles contain information in the form of chemicals, cytokines to particles of messenger RNA.
Where are Exosomes found?
Exosomes are produced from stem cells and can be found in a variety of tissues.
How are they different from Stem Cells?
Exosomes are contained in stem cells. They are messenger cells that travel to communicate with other cells to produce the needed proteins to repair damaged areas. The use of exosomes does not require donor cells to be injected in the body. Exosomes can be extracted from mesenchymal stem cells (MSCs), sterilized and then injected into the body.
Stem cell therapy injects donor cells into a specific location. These stem cells grow and divide to repair the injured area and create healthier cells overtime. Exosomes are extracted from mesenchymal stem cells and like stem cells are injected into the injured area. Exosomes get to work at communicating with the patient’s own cells and enhance communication among the cells to generate the necessary and needed cells for healing.
What Exactly is Exosome Therapy ?
Exosome products are generally used for orthopedic injuries. Much like stem cell therapy, the exosomes would be injected into the injured site. This is generally an outpatient therapy that is relatively painless.
What does Exosome Therapy Treat? (Medical applications)
Exosome therapy is used for a variety of musculoskeletal injuries, chronic pain, degenerative diseases and genetic disorders. Aging and injuries suppress your cells ability to communicate with each other. The whole purpose of exosomes is to promote communication between cells to repair damaged tissues.
Exosomes are also used to help repair the skin from effects of aging. Overtime, the dermal layer of the skin is damaged because of fragmentation in the collagen, resulting in the signs of aging and hindering the ability to heal wounds. Exosomes have been found to help the body ramp up its collagen production. Exosomes also help inhibit inflammatory cytokines. This means exosomes can help with Atopic Dermatitis, a common skin disorder.
Exosomes can also help with lyme disease. Lyme disease is a very complex disease that compromises the immune system. This can lead to the disruption of cellular health and function. Oftentimes, lyme disease patients experience difficulty with inflammation which exosomes are also known to treat.
Are Exosomes Safer than Stem Cells
While both therapies have a low risk of complications, exosome therapy does not involve a surgical procedure for harvesting cells. Stem cells’ primary job is to grow and divide new and healthy cells from the donor cells. At times these cells may rapidly multiply resulting in a tumor. Exosomes do not multiply, rather they are responsible for improving the communication among cells.
If you want to become a specialist in stem cell therapies, exosomes and MSCs with us, check our next training dates here
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Wharton’s Jelly – how does it work?
Wharton’s Jelly Treatment
Wharton’s Jelly is the substance that helps lubricate and support the umbilical cord. What makes Wharton’s Jelly so special is it contains high levels of mesenchymal stem cells. These special stem cells adapt to fit the cells needed to help regenerate damaged tissue and relieve pain naturally.
The mesenchymal stem cells that come from Wharton’s Jelly contain the most potent stem cells that are rich in regenerative properties. Wharton’s Jelly also contains a good amount of collagen, hyaluronic acid, and anti-inflammatory properties. This makes them quintessential in regenerative medicine.
Regenerative medicine refers to treatments and medicine that is naturally occuring, stem cell therapies being included. Mesenchymal stem cells are harvested from the Wharton Jelly found in the umbilical cord that has been donated by a healthy mother of a full term baby. These stem cells contain all the regenerative properties needed to help the body heal and relieve pain naturally.
How does this treatment work?
Mesenchymal stem cells are injected into the body and have the ability to transmute into cells that are needed to repair damaged tissue and relieve inflammation. These cells harness the potent regenerative properties that help rebuild the injured part of the body and provide long lasting natural pain relief. Unlike pain medicines which provide temporary relief that mask the symptoms and are harmful to the body if taken long term, the stem cells found in Wharton’s Jelly are naturally occurring and natural to the body.
What is Wharton’s Jelly best used for?
Wharton’s Jelly is best used in patients with degenerative diseases and musculoskeletal injuries. The anti-inflamatory and regenerative properties go to work at repairing damaged tissues. Repaired tissue means lasting pain relief and restored function.
What can someone expect?
Once a patient and doctor have decided on this type of stem cell treatment, patients can expect a relatively painless process. Stem cells are injected into the site and immediately go to work. Injections are virtually painless and there are no known negative side effects. Patients can generally get right back to the activities they love. Doctors will discuss treatment plans based on individual needs and in some cases resting an injured area for a particular period of time will be needed.
The mesenchymal stem cells found in Whorton’s Jelly have proven to be a very successful therapeutic method to treat a number of degenerative issues and injuries. This therapy is relatively painless, with no known negative side effects making it an excellent choice in treatment. Instead of masking the pain with dangerous medicine, stem cell therapy offers a naturally occurring solution that aims to repair the damaged tissue and resolve the problem.
If you want to learn more about Wharton’s Jelly Treatment and how can you help your patients, you can check our next training course here
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Exosomes: Everything you need to know about this new therapy
Exosomes: everything you need to know about this new therapy
Stem cell-derived exosome therapy is an effective and safe way to relieve chronic pain, leaving behind the need for invasive treatments, such as surgery or drugs with undesirable side effects.
What is exosome therapy?
Normally our cells release substances through envelope-like structures called vesicles. Exosomes are a type of vesicle produced especially by stem cells (mesenchymal cells).
In essence, these small messengers transport crucial molecules or substances, such as signaling proteins or genetic information from one cell to another in order to modulate or generate a response.
The aim of these vesicles is to enhance nerve signaling between cells to reduce the inflammatory response, while at the same time stimulating cell regeneration and modifying immune reactions to pathogens and other diseases.
Why do exosomes relieve pain and inflammation?
With aging, our reparative cells lose some of their ability to respond. Some environmental factors, genetic alterations, or chronic diseases, such as autoimmune disorders and Lyme disease can also interfere with or alter signaling between cells, leaving them unable to mediate inflammation and repair injured tissues.
The main goal of exosome therapy is to modulate both T cells and NK cells in the immune system. Both cells are critical for a balanced immune response, but when that balance is lost for multiple reasons, the result is often an immune imbalance that causes pain and chronic inflammation that is difficult to manage.
These vesicles work by regulating the activity of these cells. For example, when a joint is affected, exosomes are responsible for sending regulatory signals only to the altered cells to restore their normal function.
Therefore, exosomes play a leading role in the regulation and repair of damaged cells and tissues.
What does this new therapy consist of?
The exosomes are administered through an injectable solution that is applied to the affected area. In a single milliliter of solution, there can be between one and 16 billion exosomes. The dosage will depend on the patient’s needs and the therapeutic plan elaborated by the specialist.
Advantages of exosome therapy:
- The entire process occurs on an outpatient basis.
- It is not invasive, nor does it require anesthesia.
- It does not require previous preparations or a long period of inactivity for recovery.
- As it is a substance identical to the biological ones, it does not generate adverse reactions.
However, after treatment, it is necessary to avoid anti-inflammatory drugs, high-impact activities, or cardiovascular exercise for at least the first two weeks.
The results of the therapy depend on the patient’s age, condition, and severity of the condition. As we age our body deteriorates and responds slowly to signals from the cells, therefore in some cases the results are not immediate and several sessions may be necessary to achieve a noticeable improvement.
Where can I receive treatment with exosomes?
Currently, regenerative medicine is already being applied in different parts of the world.
In some places, the intervention and any other regenerative medicine procedure are certainly more cost-effective.
At Cellular Hope Institute we perform exosome and stem cell therapy in Cancun, Mexico. One of the most affordable and cost-effective locations for patients.
Our team will help you with:
- Scheduling therapy dates.
- Booking airline tickets.
- Providing hotel recommendations: many hotels are located next to the hospital.
- Arranging door-to-door transportation during your stay in Cancun, including assistance from and to the airport, hotel, hospital, and anywhere else you would like to go during your stay.
- Accompanying you from and to the hospital and answering any questions along the way.
- Published in Blog
